Advanced Protocol™ Verified PicoLab® Mouse 50 IF Diet is a Constant Nutrition™ formulation providing 20% protein for mouse colonies that require extra levels of energy and where dietary estrogenic activity needs to be assured. Irradiation treatment and special 4-ply packaging provide virtually bacteria-free dietary control. Especially suited for transgenic mice and breeding mice colonies maintained in barrier facilities.
| Features and Benefits |
Formulated with 20% protein |
|
Verified to contain less than 50 ppm total isoflavones (genistein, daidzein and
glycitein) |
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Reliable microbial control |
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Precision processing assures Constant Nutrition™ quality |
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Irradiation eliminates the need for autoclaving |
Product Forms Available |
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Oval pellets, 10 mm x 16 mm x 25 mm length (3/8”x5/8”x1”) |
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Meal (ground pellets), special order |
Strain differences have been shown when different dietary fat levels are fed. BALBc mice improve in breeding performance when fed higher fat levels while C57BL/6 mice tend to reduce the number born and weaned when fed a high fat diet.
Strains showing optimum reproductive performance when fed high fat diets should receive 5V03, while those not requiring high fat diets should be fed Advanced Protocol™ Verified PicoLab® Rodent 50 IF Diet - 5V02 when involved in protocols involving estrogen-sensitive parameters.
An estrogen-sensitive parameter in the growing, ovariectomized mouse is the uterus. A smaller uterus indicates less estrogenic activity in the diet. When compared on a basis relative to body weight (BW), less than 50 ppm total isoflavones produced a uterus that was 15% smaller than a uterus produced by approximately 400 ppm total isoflavones.
G U A R A N T E E D A N A L Y S I S |
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| Crude protein not less than | 20.0% |
| Crude fat not less than | 9.0% |
| Crude fiber not more than | 6.0% |
I N G R E D I E N T S
Ground wheat, ground corn, corn gluten meal, wheat middlings, ground oats, corn oil, casein, dicalcium phosphate, brewers dried yeast, calcium carbonate, L-lysine, salt, menadione sodium bisulfite (source of vitamin K), choline chloride, magnesium oxide, pyridoxine hydrochloride, DL-methionine, vitamin A acetate, cholecalciferol (source of vitamin
D3), thiamin mononitrate, dl-alpha tocopheryl acetate (source of vitamin E), calcium pantothenate, L-tryptophan, folic acid, cyanocobalamin (source of vitamin
B12), riboflavin, nicotinic acid, manganese oxide, zinc oxide, ferrous carbonate, copper sulfate, zinc sulfate, calcium iodate, cobalt carbonate, sodium selenite.
F E E D I N G D I R E C T I O N S
Feed ad libitum. Plenty of fresh, clean water should be available to the animals at all times.
Mice-Adult mice will eat 4 to 5 grams of pelleted ration daily. Some of the larger strains may eat as much as 8 grams per day per animal. Feed should be available on a free choice basis in wire feeders above the floor of the cage.
| C H E M I C A L C O M P O S I T I O N | |
| Nutrients (2) | |
| Protein, % |
20.0 |
| Arginine, % | 0.78 |
| Cystine, % | 0.27 |
| Glycine, % | 0.61 |
| Histidine, % | 0.45 |
| Isoleucine, % | 0.96 |
| Leucine, % | 2.28 |
| Lysine, % | 1.15 |
| Methionine, % | 0.51 |
| Phenylalanine, % | 1.07 |
| Tyrosine, % | 0.81 |
| Threonine, % | 0.70 |
| Tryptophan, % | 0.20 |
| Valine, % | 1.05 |
| Serine, % | 1.01 |
| Aspartic Acid, % | 1.41 |
| Glutamic Acid, % | 4.94 |
| Alanine, % | 1.26 |
| Proline, % | 1.98 |
| Taurine, % | 0.00 |
| Fat (ether extract), % | 9.0 |
| Fat (acid hydrolysis), % | 10.2 |
| Cholesterol, ppm | 0.00 |
| Linoleic Acid, % | 5.33 |
| Linolenic Acid, % | 0.14 |
| Arachidonic Acid, % | 0.00 |
| Omega-3 Fatty Acids, % | 0.14 |
| Total Saturated Fatty Acids, % | 1.41 |
| Total
Monounsaturated Fatty Acids, % |
2.30 |
| Fiber (Crude), % | 3.4 |
| Neutral Detergent Fiber3, % | 14.5 |
| Acid Detergent Fiber4, % | 4.9 |
| Nitrogen-Free Extract (by difference), % |
57.4 |
| Starch, % |
37.9 |
| Glucose, % |
0.16 |
| Fructose, % |
0.15 |
| Sucrose, % |
0.34 |
| Lactose, % |
0.00 |
| Total Digestible Nutrients, % |
80.7 |
| Gross Energy, kcal/gm |
4.28 |
| Physiological
Fuel Value5, kcal/gm |
3.71 |
| Metabolizable Energy, kcal/gm |
3.47 |
| Minerals | |
| Ash, % |
5.2 |
| Calcium, % |
1.00 |
| Phosphorus, % |
0.80 |
| Phosphorus (non-phytate), % |
0.58 |
| Potassium, % |
0.36 |
| Magnesium, % |
0.20 |
| Sulfur, % |
0.16 |
| Sodium, % |
0.23 |
| Chlorine, % |
0.52 |
| Fluorine, ppm |
3.7 |
| Iron, ppm |
140 |
| Zinc, ppm |
110 |
| Manganese, ppm |
140 |
| Copper, ppm |
15 |
| Cobalt, ppm |
0.51 |
| Iodine, ppm |
1.5 |
| Chromium, ppm |
0.34 |
| Selenium, ppm |
0.30 |
| Vitamins | |
| Carotene, ppm |
1.9 |
| Vitamin K (as menadione), ppm |
7.1 |
| Thiamin Hydrochloride, ppm |
27 |
| Riboflavin, ppm |
8.9 |
| Niacin, ppm |
91 |
| Pantothenic Acid, ppm |
30 |
| Choline Chloride, ppm |
2200 |
| Folic Acid, ppm |
2.7 |
| Pyridoxine, ppm |
11 |
| Biotin, ppm |
0.13 |
| B12, mcg/kg |
43 |
| Vitamin A, IU/gm |
25 |
| Vitamin D3 (added), IU/gm |
2.0 |
| Vitamin E, IU/kg |
94 |
| Ascorbic Acid, mg/gm |
-- |
| Calories provided by: | |
| Protein, % | 21.587 |
| Fat (ether extract), % | 21.856 |
| Carbohydrates, % | 56.557 |
* Product Code
1. Based on the latest ingredient analysis information. Since nutrient composition
of natural ingredients varies, analysis will differ accordingly.
2. Nutrients expressed as percent of ration except where otherwise indicated.
3. NDF = approximately cellulose, hemi-cellulose and lignin.
4. ADF = approximately cellulose and lignin.
5. Physiological Fuel Value (kcal/gm) = Sum of decimal fractions of protein, fat and
carbohydrate (use Nitrogen Free Extract) x 4,9,4 kcal/gm respectively.
02/17/03