Advanced Protocol™ Verified 150 IF Diet is a Constant Nutrition™ formulation providing 20% protein for rats (and mice) in protocols where moderate dietary estrogenic activity is a concern.

Features and Benefits
blkdot.gif (828 bytes) Formulated with 20% protein
blkdot.gif (828 bytes) Verified to contain less than 150 ppm total isoflavones (genistein, daidzein and glycitein)
blkdot.gif (828 bytes) Provides proper nutrients without affecting outcome in estrogen-sensitive protocols as shown in the accompanying graph
blkdot.gif (828 bytes) Precision processing assures Constant Nutrition™ quality

Product Forms Available
blkdot.gif (828 bytes) Oval pellets, 10 mm x 16 mm x 25 mm length (3/8”x5/8”x1”)
blkdot.gif (828 bytes) Meal (ground pellets), special order

Rat Strain Reproductive Performance

Born/Pair 5001 5V00 5V01
Inbred 24 20 30
Outbred 39 44 39
Weaned/Pair
Inbred 18 a,b 12 a 22 b
Outbred 25 26 25

G U A R A N T E E D     A N A L Y S I S
Crude protein not less than

20.0%

Crude fat not less than

4.5%

Crude fiber not more than

6.0%

I N G R E D I E N T S
Ground wheat, corn gluten meal, wheat middlings, ground corn, ground oats, dried beet pulp, wheat germ, cane molasses, dried whey, soy protein concentrate, corn oil, calcium carbonate, dehulled soybean meal, dicalcium phosphate, casein, salt, L-lysine, menadione dimethylpyrimidinol bisulfite (source of vitamin K), choline chloride, pyridoxine hydrochloride, potassium chloride, chromium potassium sulfate, DL-methionine, cholecalciferol (source of vitamin D3), L-tryptophan, vitamin A acetate, dl-alpha tocopheryl acetate (source of vitamin E), cyanocobalamin (source of vitamin B12), folic acid, thiamin mononitrate, ferrous sulfate, biotin, nicotinic acid, calcium pantothenate, riboflavin, zinc oxide, manganous oxide, ferrous carbonate, copper sulfate, zinc sulfate, calcium iodate, cobalt carbonate, sodium selenite.

F E E D I N G    D I R E C T I O N S
Feed ad libitum. Plenty of fresh, clean water should be available to the animals at all times. 
Rats- All rats will eat varying amounts of feed depending on their genetic origin. Larger strains will eat between 15-30 grams per day. Smaller strains will eat between 12-15 grams per day. Feeders in rat cages should be designed to hold two to three days supply of feed at one time. 
Mice-Adult mice will eat 4 to 5 grams of pelleted ration daily. Some of the larger strains may eat as much as 8 grams per day per animal. Feed should be available on a free choice basis in wire feeders above the floor of the cage. 
Hamsters-Adults will eat 10 to 14 grams per day. 

C H E M I C A L    C O M P O S I T I O N
Nutrients (2)
Protein, %

20.0

Arginine, % 0.98
Cystine, % 0.37
Glycine, % 0.68
Histidine, % 0.44
Isoleucine, % 0.97
Leucine, % 2.17
Lysine, % 1.00
Methionine, % 0.43
Phenylalanine, % 1.08
Tyrosine, % 0.75
Threonine, % 0.75
Tryptophan, % 0.24
Valine, % 1.00
Serine, % 1.01
Aspartic Acid, % 1.55
Glutamic Acid, % 5.05
Alanine, % 1.17
Proline, % 1.85
Taurine, % 0.00
Fat (ether extract), % 4.5
Fat (acid hydrolysis), % 5.6
Cholesterol, ppm 0.00
Linoleic Acid, % 2.74
Linolenic Acid, % 0.12
Arachidonic Acid, % 0.00
Omega-3 Fatty Acids, % 0.12
Total Saturated Fatty Acids, % 0.84
Total Monounsaturated
Fatty Acids, %
1.17
Fiber (Crude), % 4.2
Neutral Detergent Fiber3, % 15.9
Acid Detergent Fiber4, % 5.9
Nitrogen-Free Extract (by difference), %

55.4

Starch, %

33.6

Glucose, %

0.08

Fructose, %

0.08

Sucrose, %

2.60

Lactose, %

2.57

Total Digestible Nutrients, %

76.0

Gross Energy, kcal/gm

4.01

Physiological Fuel
Value5, kcal/gm

3.42

Metabolizable Energy, kcal/gm

3.17

  
Minerals
Ash, %

5.9

Calcium, %

0.96

Phosphorus, %

0.60

Phosphorus (non-phytate), %

0.36

Potassium, %

0.74

Magnesium, %

0.18

Sulfur, %

0.25

Sodium, %

0.30

Chlorine, %

0.57

Fluorine, ppm

3.1

Iron, ppm

180

Zinc, ppm

130

Manganese, ppm

120

Copper, ppm

15

Cobalt, ppm

0.73

Iodine, ppm

1.49

Chromium, ppm

0.84

Selenium, ppm

0.30

      
Vitamins
Carotene, ppm

1.7

Vitamin K (as menadione), ppm

4.0

Thiamin Hydrochloride, ppm

13

Riboflavin, ppm

7.9

Niacin, ppm

91

Pantothenic Acid, ppm

17

Choline Chloride, ppm

1700

Folic Acid, ppm

2.6

Pyridoxine, ppm

9.2

Biotin, ppm

0.23

B12, mcg/kg

70

Vitamin A, IU/gm

9.9

Vitamin D3 (added), IU/gm

2.2

Vitamin E, IU/kg

74

Ascorbic Acid, mg/gm

--

   
Calories provided by:
Protein, % 23.385
Fat (ether extract), % 11.839
Carbohydrates, % 64.776

*   Product Code
1.  Based on the latest ingredient analysis information. Since nutrient composition of natural ingredients varies, analysis will differ accordingly.
2.  Nutrients expressed as percent of ration except where otherwise indicated.
3.  NDF = approximately cellulose, hemi-cellulose and lignin.
4.  ADF = approximately cellulose and lignin.
5.  Physiological Fuel Value (kcal/gm) = Sum of decimal fractions of protein, fat and carbohydrate (use Nitrogen Free Extract) x 4,9,4 kcal/gm respectively.

02/17/03