Advanced Protocol™ Verified 50 IF is a Constant Nutrition™ formulation providing 20% protein for mice (and rats) in protocols where low dietary estrogenic activity needs to be assured.
| Features and Benefits |
Formulated with 20% protein |
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Verified to contain less than 50 ppm total isoflavones (genistein, daidzein and
glycitein) |
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Provides proper nutrients without affecting outcome in estrogen-sensitive protocols as shown in the accompanying graph |
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Precision processing assures Constant Nutrition™ quality |
Product Forms Available |
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Oval pellets, 10 mm x 16 mm x 25 mm length (3/8”x5/8”x1”) |
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Meal (ground pellets), special order |
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Growing OVX Mouse Uterus |
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| An estrogen-sensitive parameter in the growing, ovariectomized mouse is the uterus. A smaller uterus indicates less estrogenic activity in the diet. When compared on a basis relative to body weight (BW), 5V00 (less than 50 ppm total isoflavones) produced a uterus that was 15% smaller than a uterus produced by LabDiet 5001 (approximately 400 ppm total isoflavones). |
G U A R A N T E E D A N A L Y S I S |
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| Crude protein not less than | 20.0% |
| Crude fat not less than | 4.5% |
| Crude fiber not more than | 6.0% |
I N G R E D I E N T S
Ground wheat, ground corn, ground oats, wheat middlings, corn gluten meal, fish meal, casein, corn oil, dicalcium phosphate, L-lysine, calcium carbonate, dehulled soybean meal, salt, menadione sodium bisulfite (source of vitamin K), choline chloride, magnesium oxide, pyridoxine hydrochloride, potassium chloride, vitamin A acetate, DL-methionine, cholecalciferol (source of vitamin
D3), thiamin mononitrate, dl-alpha tocopheryl acetate (source of vitamin E), calcium pantothenate, L-tryptophan, folic acid, manganese oxide, riboflavin, cyanocobalamin (source of vitamin
B12), nicotinic acid, biotin, zinc oxide, chromium potassium sulfate, ferrous carbonate, copper sulfate, zinc sulfate, calcium iodate, cobalt carbonate, sodium selenite.
F E E D I N G D I R E C T I O N S
Feed ad libitum. Plenty of fresh, clean water should be available to the animals at all times.
Rats- All rats will eat varying amounts of feed depending on their genetic origin. Larger strains will eat between 15-30 grams per day. Smaller strains will eat between 12-15 grams per day. Feeders in rat cages should be designed to hold two to three days supply of feed at one time.
Mice-Adult mice will eat 4 to 5 grams of pelleted ration daily. Some of the larger strains may eat as much as 8 grams per day per animal. Feed should be available on a free choice basis in wire feeders above the floor of the cage.
Hamsters-Adults will eat 10 to 14 grams per day.
| C H E M I C A L C O M P O S I T I O N | |
| Nutrients (2) | |
| Protein, % |
20.0 |
| Arginine, % | 0.89 |
| Cystine, % | 0.25 |
| Glycine, % | 0.77 |
| Histidine, % | 0.44 |
| Isoleucine, % | 0.97 |
| Leucine, % | 1.87 |
| Lysine, % | 1.00 |
| Methionine, % | 0.48 |
| Phenylalanine, % | 0.94 |
| Tyrosine, % | 0.64 |
| Threonine, % | 0.68 |
| Tryptophan, % | 0.22 |
| Valine, % | 1.00 |
| Serine, % | 0.93 |
| Aspartic Acid, % | 1.46 |
| Glutamic Acid, % | 4.59 |
| Alanine, % | 1.13 |
| Proline, % | 1.74 |
| Taurine, % | 0.02 |
| Fat (ether extract), % | 4.5 |
| Fat (acid hydrolysis), % | 5.5 |
| Cholesterol, ppm | 160 |
| Linoleic Acid, % | 2.29 |
| Linolenic Acid, % | 0.10 |
| Arachidonic Acid, % | 0.01 |
| Omega-3 Fatty Acids, % | 0.24 |
| Total Saturated Fatty Acids, % | 0.94 |
| Total
Monounsaturated Fatty Acids, % |
1.13 |
| Fiber (Crude), % | 3.5 |
| Neutral Detergent Fiber3, % | 15.0 |
| Acid Detergent Fiber4, % | 4.7 |
| Nitrogen-Free Extract (by difference), % |
56.3 |
| Starch, % |
41.5 |
| Glucose, % |
0.16 |
| Fructose, % |
0.16 |
| Sucrose, % |
0.38 |
| Lactose, % |
0.00 |
| Total Digestible Nutrients, % |
74.9 |
| Gross Energy, kcal/gm |
4.02 |
| Physiological
Fuel Value5, kcal/gm |
3.45 |
| Metabolizable Energy, kcal/gm |
3.18 |
| Minerals | |
| Ash, % |
5.7 |
| Calcium, % |
1.06 |
| Phosphorus, % |
0.80 |
| Phosphorus (non-phytate), % |
0.58 |
| Potassium, % |
0.44 |
| Magnesium, % |
0.20 |
| Sulfur, % |
0.20 |
| Sodium, % |
0.26 |
| Chlorine, % |
0.71 |
| Fluorine, ppm |
6.7 |
| Iron, ppm |
164 |
| Zinc, ppm |
109 |
| Manganese, ppm |
130 |
| Copper, ppm |
11 |
| Cobalt, ppm |
0.58 |
| Iodine, ppm |
1.1 |
| Chromium, ppm |
0.99 |
| Selenium, ppm |
0.30 |
| Vitamins | |
| Carotene, ppm |
1.1 |
| Vitamin K (as menadione), ppm |
7.1 |
| Thiamin Hydrochloride, ppm |
27 |
| Riboflavin, ppm |
8.5 |
| Niacin, ppm |
85 |
| Pantothenic Acid, ppm |
29 |
| Choline Chloride, ppm |
1750 |
| Folic Acid, ppm |
2.7 |
| Pyridoxine, ppm |
11 |
| Biotin, ppm |
0.13 |
| B12, mcg/kg |
45 |
| Vitamin A, IU/gm |
25 |
| Vitamin D3 (added), IU/gm |
2.0 |
| Vitamin E, IU/kg |
93 |
| Ascorbic Acid, mg/gm |
-- |
| Calories provided by: | |
| Protein, % | 23.141 |
| Fat (ether extract), % | 11.715 |
| Carbohydrates, % | 65.144 |
* Product Code
1. Based on the latest ingredient analysis information. Since nutrient composition
of natural ingredients varies, analysis will differ accordingly.
2. Nutrients expressed as percent of ration except where otherwise indicated.
3. NDF = approximately cellulose, hemi-cellulose and lignin.
4. ADF = approximately cellulose and lignin.
5. Physiological Fuel Value (kcal/gm) = Sum of decimal fractions of protein, fat and
carbohydrate (use Nitrogen Free Extract) x 4,9,4 kcal/gm respectively.
07/23/03
