Prolab® RMH 2500, Autoclavable 5R24 is an autoclavable version of 5P14. It is a complete and balanced diet manufactured under controlled formulation to assure Constant Nutrition™. This diet is an all-around rodent diet designed for laboratory rats, mice and hamsters, in a wide range of applications, including research studies and non-intensive reproduction in situations where an autoclavable feed is desired. Refer to the Shelf Life Section on this site for product longevity information and storage suggestions.

Features and Benefits
blkdot.gif (828 bytes) Utilizes a wide range of energy sources to develop nutrition at economical cost
blkdot.gif (828 bytes) Processed and packaged for autoclaving
blkdot.gif (828 bytes) Produced under Constant Nutrition™
   
Product Forms Available
blkdot.gif (828 bytes) Oval pellet, 10 mm x 16 mm x 25 mm length (3/8"x5/8"x1")

G U A R A N T E E D     A N A L Y S I S
Crude protein not less than

23.0%

Crude fat not less than

4.5%

Crude fiber not more than

6.0%

Ash not more than

8.0%

Added minerals not more than

2.5%

A U T O C L A V I N G    S U G G E S T I O N S
To autoclave the pellets, place on trays, in small bags, or in larger bags, to a depth of no more than 3 inches. When steam autoclaved, the pellets swell and exert force on adjacent pellets. Confinement by a bag or container creates additional pressure, which may result in sticking. Assay before and after autoclaving:   Conditions of sterilization must be determined for each autoclaving unit. Microbiological evaluation should be done to insure sterilization is achieved. It is best to assay the diet before and after sterilization to determine nutrient losses.

I N G R E D I E N T S
Dehulled soybean meal, ground corn, wheat middlings, ground oats, cane molasses, dehydrated alfalfa meal, fish meal, animal fat preserved with BHA, calcium carbonate, porcine meat meal, ground wheat, dried beet pulp, salt, wheat germ, dried whey, soybean hulls, dicalcium phosphate, corn gluten meal, silicon dioxide, menadione dimethylpyrimidinol bisulfite (source of vitamin K), thiamin mononitrate, choline chloride, soybean oil, cholecalciferol, vitamin A acetate, brewers dried yeast, folic acid, pyridoxine hydrochloride, DL-methionine, calcium pantothenate, dl-alpha tocopheryl acetate, nicotinic acid, riboflavin, cyanocobalamin, biotin, ferrous sulfate, manganous oxide, zinc oxide, ferrous carbonate, copper sulfate, zinc sulfate, calcium iodate, cobalt carbonate, sodium selenite.

F E E D I N G    D I R E C T I O N S
Feed ad libitum to rodents. Plenty of fresh, clean water should be available to the animals at all times. Refer to the “Animal Care and Biological Values” section of this manual for detailed feeding directions.
Rats- All rats will eat varying amounts of feed depending on their genetic origin. Larger strains will eat between 15-30 grams per day. Smaller strains will eat between 12-15 grams per day. Feeders in rat cages should be designed to hold two to three days supply of feed at one time.
Mice-Adult mice will eat 4 to 5 grams of pelleted ration daily. Some of the larger strains may eat as much as 8 grams per day per animal. Feed should be available on a free choice basis in wire feeders above the floor of the cage.
Hamsters-Adults will eat 10 to 14 grams per day.

C H E M I C A L    C O M P O S I T I O N 1
Nutrients 2
Protein, %

24.9
Arginine, % 1.62
Cystine, % 0.35
Glycine, % 1.20
Histidine, % 0.63
Isoleucine, % 1.25
Leucine, % 2.00
Lysine, % 1.45
Methionine, % 0.49
Phenylalanine, % 1.18
Tyrosine, % 0.77
Threonine, % 0.97
Tryptophan, % 0.32
Valine, % 1.31
Serine, % 1.37
Aspartic Acid, % 2.90
Glutamic Acid, % 5.35
Alanine, % 1.33
Prolline, % 1.72
Taurine, % 0.00
Fat (ether extract), % 4.8
Fat (acid hydrolysis), % 6.3
Cholesterol, ppm 97
Linoleic Acid, % 1.77
Linolenic Acid, % 0.14
Arachidonic Acid, % 0.00
Omega-3 Fatty Acids, % 0.20
Total Saturated Fatty Acids, % 1.54
Total Monounsaturated Fatty Acids, % 1.31
Fiber (Crude), % 4.7
Neutral Detergent Fiber3, % 13.7
Acid Detergent Fiber4, % 5.6
Nitrogen-Free Extract (by difference), %

49.3
Starch, % 25.1
Glucose, % 0.2
Fructose, % 0.2
Sucrose, % 2.1
Lactose, % 0.3
Total Digestible Nutrients, %

77.5
Gross Energy, kcal/gm

4.10
Physiological Fuel Value5, kcal/gm

3.40
Metabolizable Energy, kcal/gm

3.12
  
Minerals
Ash, %

6.3
Calcium, %

1.00
Phosphorus, %

0.62
Phosphorus (non-phytate), %

0.27
Potassium, %

1.12
Magnesium, %

0.22
Sulfur, %

0.28
Sodium, %

0.29
Chlorine, %

0.50
Fluorine, ppm

10
Iron, ppm

310
Zinc, ppm

150
Manganese, ppm

140
Copper, ppm

21
Cobalt, ppm

0.60
Iodine, ppm

2.0
Chromium, ppm

1.2
Selenium, ppm

0.32
      
Vitamins
Carotene, ppm

2.3
Vitamin K (as menadione), ppm

3.2
Thiamin Hydrochloride, ppm

91
Riboflavin, ppm

15
Niacin, ppm

130
Pantothenic Acid, ppm

26
Choline Chloride, ppm

2200
Folic Acid, ppm

7.9
Pyridoxine, ppm

17
Biotin, ppm

0.35
B12, mcg/kg

33
Vitamin A, IU/gm

44
Vitamin D3 (added), IU/gm

4.5
Vitamin E, IU/kg

67
Ascorbic Acid, mg/gm

--
   
Calories provided by:
Protein, % 29.294
Fat (ether extract), % 12.706
Carbohydrates, % 58.000

*   Product Code
1.  Based on the latest ingredient analysis information. Since nutrient composition of natural ingredients varies, analysis will differ accordingly.
2.  Nutrients expressed as percent of ration except where otherwise indicated.   Moisture content is assumed to be 10.0% for the purpose of calculations.
3.  NDF = approximately cellulose, hemi-cellulose and lignin.
4.  ADF = approximately cellulose and lignin.
5.  Physiological Fuel Value (kcal/gm) = Sum of decimal fractions of protein, fat and carbohydrate (use Nitrogen Free Extract) x 4,9,4 kcal/gm respectively.

07/01/04