Advanced Protocol™ Certified Rodent Diet, 18% is a Constant Nutrition™ formulation for the maintenance, growth and reproduction of rats and mice. A sample of this product will have been assayed prior to shipment. Nutritional quality will be maintained for six months when stored at 72°F (22°C) with 50% relative humidity. For protocols that call for 5002 with lower protein.

Features and Benefits
blkdot.gif (828 bytes) Each package is assayed for environmental contaminants prior to shipment
blkdot.gif (828 bytes) Preanalysis monitoring assures maximum diet control
blkdot.gif (828 bytes) Certification profile fulfills GLP requirements
   
Product Forms Available
blkdot.gif (828 bytes) Oval Pellet, 10 mm x 16 mm x 25 mm length (3/8"x5/8"x1")
blkdot.gif (828 bytes) Meal (ground pellets)

G U A R A N T E E D     A N A L Y S I S
Crude protein not less than

18.0%

Crude fat not less than

3.5%

Crude fiber not more than

6.0%

I N G R E D I E N T S
Ground corn, ground wheat, dehulled soybean meal, wheat middlings, dried beet pulp, brewers dried yeast, dehydrated alfalfa meal, soybean oil, calcium carbonate, salt, dicalcium phosphate, L-lysine, menadione dimethylpyrimidinol bisulfite (source of vitamin K), choline chloride, DL-methionine, potassium chloride, pyridoxine hydrochloride, cholecalciferol (source of vitamin D3), dl-alpha tocopheryl acetate (source of vitamin E), vitamin A acetate, folic acid, biotin, nicotinic acid, calcium pantothenate, riboflavin, cyanocobalamin (source of vitamin B12), thiamin mononitrate, manganous oxide, zinc oxide, ferrous carbonate, copper sulfate, zinc sulfate, calcium iodate, cobalt carbonate, sodium selenite.

F E E D I N G    D I R E C T I O N S
Feed ad libitum to rodents. Plenty of fresh, clean water should be available to the animals at all times. Refer to the "Animal Care and Biological Values" section of this manual for detailed feeding directions.
Rats-All rats will eat varying amounts of feed depending on their genetic origin. Larger strains will eat between 15-30 grams per day. Smaller strains will eat between 12-15 grams per day. Feeders in rat cages should be designed to hold two to three days supply of feed at one time.
Mice-Adult mice will eat 4 to 5 grams of pelleted ration daily. Some of the larger strains may eat as much as 8 grams per day per animal. Feed should be available on a free choice basis in wire feeders above the floor of the cage.

C H E M I C A L    C O M P O S I T I O N 1
Nutrients 2
Protein, %

18.0

Arginine, % 1.05
Cystine, % 0.26
Glycine, % 0.79
Histidine, % 0.43
Isoleucine, % 0.89
Leucine, % 1.41
Lysine, % 1.06
Methionine, % 0.35
Phenylalanine, % 0.82
Tyrosine, % 0.49
Threonine, % 0.65
Tryptophan, % 0.24
Valine, % 0.88
Serine, % 1.02
Aspartic Acid, % 2.07
Glutamic Acid, % 4.44
Alanine, % 1.02
Prolline, % 1.45
Taurine, % 0.00
Fat (ether extract), % 3.5
Fat (acid hydrolysis), % 4.4
Cholesterol, ppm 0.00
Linoleic Acid, % 1.89
Linolenic Acid, % 0.20
Arachidonic Acid, % 0.00
Omega-3 Fatty Acids, % 0.20
Total Saturated Fatty Acids, % 0.67
Total Monounsaturated Fatty Acids, % 0.73
Fiber (Crude), % 4.0
Neutral Detergent Fiber3, % 15.3
Acid Detergent Fiber4, % 5.7
Nitrogen-Free Extract (by difference), %

59.7

Starch, % 46.5
Glucose, % 0.2
Fructose, % 0.3
Sucrose, % 1.3
Lactose, % 0.00
Total Digestible Nutrients, %

77.4

Gross Energy, kcal/gm

3.99

Physiological Fuel Value5, kcal/gm

3.42

Metabolizable Energy, kcal/gm

2.86

  
Minerals
Ash, %

4.8

Calcium, %

0.75

Phosphorus, %

0.56

Phosphorus (non-phytate), %

0.30

Potassium, %

0.82

Magnesium, %

0.18

Sulfur, %

0.21

Sodium, %

0.26

Chlorine, %

0.50

Fluorine, ppm

12

Iron, ppm

200

Zinc, ppm

78

Manganese, ppm

73

Copper, ppm

11

Cobalt, ppm

0.52

Iodine, ppm

0.83

Chromium, ppm

1.0

Selenium, ppm

0.29

      
Vitamins
Carotene, ppm

1.3

Vitamin K (as menadione), ppm

3.3

Thiamin Hydrochloride, ppm

9.5

Riboflavin, ppm

8.0

Niacin, ppm

130

Pantothenic Acid, ppm

17

Choline Chloride, ppm

1800

Folic Acid, ppm

2.9

Pyridoxine, ppm

8.0

Biotin, ppm

0.25

B12, mcg/kg

25

Vitamin A, IU/gm

10

Vitamin D3 (added), IU/gm

2.2

Vitamin E, IU/kg

110

Ascorbic Acid, mg/gm

--

   
Calories provided by:
Protein, % 21.034
Fat (ether extract), % 9.203
Carbohydrates, % 69.763

*   Product Code
1.  Based on the latest ingredient analysis information. Since nutrient composition of natural ingredients varies, analysis will differ accordingly.
2.  Nutrients expressed as percent of ration except where otherwise indicated.   Moisture content is assumed to be 10.0% for the purpose of calculations.
3.  NDF = approximately cellulose, hemi-cellulose and lignin.
4.  ADF = approximately cellulose and lignin.
5.  Physiological Fuel Value (kcal/gm) = Sum of decimal fractions of protein, fat and carbohydrate (use Nitrogen Free Extract) x 4,9,4 kcal/gm respectively.

02/17/03